TITLE: Movie EV3

DESCRIPTION:
Tomographic sections and modeling of part of the bud neck region in a cell with Vps10-FRB-containing vesicles captured by an FKBP-tagged septin. A log-phase culture of cells expressing Vps10-FRB and Shs1-FKBP was treated for 5 min with rapamycin prior to cryopreservation and processing for cryo-ET. The first third of the movie shows every fifth section of the SIRT-reconstructed tomogram. The second third of the movie shows the same tomographic sections after contours were segmented to mark the cell cortex (yellow), two secretory vesicles (blue), putatively tethered non-secretory vesicles (bright green), and other non-secretory vesicles (dull green). Also marked are a mitochondrion (cyan) and the nuclear envelope (magenta). The final third of the movie shows a rotation of the tomographic model. Scale bar, 250 nm.

METADATA:
- Related Manuscript Title: Functional assignment of Golgi-associated vesicle tethers to specific membrane recycling pathways
- Authors: Adam H. Krahn, Natalie Johnson, Jotham Austin II, and Benjamin S. Glick
- File Format: .mp4